calculate how much bsa you need to weigh out to make 20 ml of a 1 bsa solution

The basic equation for figuring out how to make dilutions is: C1V1 = C2V2. Almost all of them are either conversions or dilutions, so two basic kinds of equations will solve all your problems. You're given an amount in micrograms and you need to convert that to microliters. If the answer is 0.10 μl, you don't need to write 0.100 μl, because you don't set the pipet that way. Do not, for example, mix 250 ml of concentrated solution with 1 liter of solvent to make a 1-liter solution. You might see this presented as a quiz or lab final question. Mix the solution with the glass stirring rod. You'll need to load 300 ng in one lane of the gel (50 ng + 250 ng). You could also use molecular mass (grams/mole) to calculate the mass of a single molecule: 1 Dalton = 1 gram/mole = 1.66 x 10-24 grams/molecule Once you have the molecular weight in Daltons, you can get the mass per molecule by dividing the molecular mass in Daltons by Avogadro’s number (6.02 x 1023 molecules/mole) or by multiplying by the reciprocal of Avogadro’s number, which is 1.66 x 10-24 mole/molecule. Calculate the molarity of the glucose solution you made in Q1. In the 6B lab, you should normally multiply the calculated amount of restriction enzyme by 10 to make sure you have enough. I encourage you to explore these; you can use them in lab, but you can't use them on quizzes or exams.
Daltons are the standard way of expressing the sizes of proteins. For a restriction digest, you'll also need to add some restriction enzyme, buffer, and perhaps some water. Many students want to reach for the calculator first, without writing out the equation; often, this leads to the wrong answer. When you do a restriction digest, the smaller bands (shorter DNA fragments) are going to contain a lower mass of DNA, so the ability to see your smallest band will be a limiting factor for your experiment. combined volume of all of the initial dilutions. If a double-stranded DNA fragment is 1200 nucleotides long, we could call it 1200 bp or 1.2 kb (kilobase pairs). It's a common mistake to add too much solvent when making the dilution. make 100 ml of a 1x solution? amount of initial volumes to be used. Write down the desired dilution in the form of a proportion--for example, 1:20 dilution, also known as the dilution factor. 0.5% means 0.5 grams in 100 ml, so if you only need 50 ml, you need 0.5 g / Edwards is a scuba instructor and Usui and Karuna Reiki teacher. If a solution of DNA is 3 mg/ml, 0.5 mg of DNA would be contained in how You'll need to do a lot of calculations in Bio 6B. As an example, your calculation might look like this: You want to solve for V1, the initial volume; this is the amount you'll add to the 4 ml of LB broth that's already in the tube. Here's how to solve it. 2 = 0.25 g agarose for a 50 ml gel solution. But you need 500 ml, final volume, so 10 g x 5 = 50 g NaCl. Since the concentration, 10x is divided by 10 to arrive at a 1x need to make up a 50 ml gel solution? Using the DNA concentration above. concentrations: 0.5% Nonidet, 150 mM Tris-Cl, and 10 mM EDTA? Protein concentration [BSA] = 40 ug / ml. Is the answer Arginase since it’s between 9-10. µl of DNA at a concentration of 4 µg/µl? Answer: There are at least two methods for solving this question When you perform DNA electrophoresis, you want to load an appropriate amount of DNA so you can clearly see the bands on the gel.

You could do it like this instead: The answer is V1=4 μl. a 0.5x solution? you can make a stock solution that is highly concentrated. If you're making this solution in the lab, you would pipet 0.2 ml (200 μl) of your starting DNA solution into a micro tube, then add 0.8 ml of water or another solution to bring the total volume up to 1 ml.
1. A dilution solution contains solute (or stock solution) and a solvent (called diluent). Instead, most DNA and RNA molecules are simpy described in terms of base pairs (for double-stranded) or nucleotides (for single-stranded). if you put 2g in 10ml water, this is a 200mg/ml solution. You need a low final concentration of amp in your culture (100 μg/ml), and it comes as a highly concentrated solution (100 mg/ml). These just do numbers, not the units. BSA Calculator.